首页> 美国卫生研究院文献>Journal of Bacteriology >Note: Occurrence of Free d-Amino Acids and Aspartate Racemases in Hyperthermophilic Archaea
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Note: Occurrence of Free d-Amino Acids and Aspartate Racemases in Hyperthermophilic Archaea

机译:注意:嗜热古生菌中游离d-氨基酸和天冬氨酸消旋物的出现

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摘要

The occurrence of free d-amino acids and aspartate racemases in several hyperthermophilic archaea was investigated. Aspartic acid in all the hyperthermophilic archaea was highly racemized. The ratio of d-aspartic acid to total aspartic acid was in the range of 43.0 to 49.1%. The crude extracts of the hyperthermophiles exhibited aspartate racemase activity at 70°C, and aspartate racemase homologous genes in them were identified by PCR. d-Enantiomers of other amino acids (alanine, leucine, phenylalanine, and lysine) in Thermococcus strains were also detected. Some of them might be by-products of aspartate racemase. It is proven that d-amino acids are produced in some hyperthermophilic archaea, although their function is unknown.
机译:研究了几种嗜热古细菌中游离d-氨基酸和天冬氨酸消旋体的存在。在所有嗜热古菌中,天冬氨酸高度消旋。 d-天冬氨酸与总天冬氨酸的比率在43.0至49.1%的范围内。超嗜热菌的粗提物在70℃下表现出天冬氨酸消旋酶活性,并通过PCR鉴定了其中的天冬氨酸消旋酶同源基因。还检测到了热球菌菌株中其他氨基酸(丙氨酸,亮氨酸,苯丙氨酸和赖氨酸)的d对映异构体。其中一些可能是天冬氨酸消旋酶的副产物。业已证明,尽管某些功能未知,但在某些嗜热古细菌中仍会产生d-氨基酸。

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