Generation of cortical neurons through large-scale expanding neuroepithelial stem cell from human pluripotent stem cells

摘要

The CHbFSB+LIF and CHbFSB support suspension expansion of NESCs. a Schematic representation of the induction and long-term suspension expansion of NESCs. b Phase-contrast of NESC-spheres from day 0 to day 3 in the CHbFSB+LIF and CHbFSB media, respectively. c The diameter dynamics of NESC-spheres over culture. Each dot visually represents a sphere. d The diameters of NESC-spheres during serial passages. e Viability dynamics of different passaged NESC-spheres (n = 3 independent experiments). Data are represented as mean ± SD. f The growth curve of NESCs growing in the CHbFSB+LIF and CHbFSB, displaying the prospect of exponential growth over serial passages. Scale bars, 200 μm. D, day; P, passage