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Rapid Profiling of the Volatilome of Cooked Meat by PTR-ToF-MS: Characterization of Chicken Turkey Pork Veal and Beef Meat

机译:PTR-TOF-MS的熟肉挥发物的迅速剖析:鸡火鸡猪肉小牛肉和牛肉的特征

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摘要

This study aimed to compare the volatile organic compound (VOC) profiles of cooked meat from different species. Four burgers were prepared and cooked from each of 100 meat samples obtained from 100 animals of five species/categories (chicken, turkey, pork, veal and beef) sourced from five supermarkets and five local butchers. Two burgers were cooked in a water bath and two were grilled. Direct proton-transfer-reaction time-of-flight mass-spectrometry (PTR-ToF-MS) analysis of the sample headspace yielded 129 mass peaks, 64 of which were tentatively identified. The results showed that turkey and chicken had the largest and the smallest total concentrations of all VOCs, respectively. Of the mammalian meats, veal and beef had greater total VOC concentrations than pork. The proportions of the amounts of all the individual VOCs differed significantly according to species. Additionally, 14 of 17 independent latent explanatory factors (LEFs) identified by multivariate analysis exhibited significant differences between meat species/categories, and therefore helped to characterize them. PTR-ToF-MS has been used for the first time for the rapid and non-invasive profiling of cooked meat of different species/categories. Knowledge of specific VOC profiles paves new avenues for research aimed at characterizing species through sensory description, at authenticating species or at identifying abnormalities or fraud.
机译:本研究旨在将熟肉的挥发性有机化合物(VOC)曲线与不同物种的挥发性有机化合物进行比较。从100种五种动物的100只动物中获得的100种肉样品中的每一个烹制了四个汉堡,从五个物种/分类(鸡,土耳其,猪肉,小牛肉和牛肉),来自五个超市和五个当地屠夫。两个汉堡在水浴中煮熟,两人烤。样品顶空间的直接质子转移反应飞行时间质谱(PTR-TOF-MS)分析产生129个质量峰,其中64个暂定鉴定。结果表明,土耳其和鸡的所有VOC的总浓度分别最大,最小。哺乳动物的肉类,小牛肉和牛肉比猪肉更大的总体浓度。根据物种,所有单独VOC的量的比例差异显着。另外,通过多变量分析鉴定的17个独立潜在的解释因素(LEF)中的14个,肉类/类别之间存在显着差异,因此有助于表征它们。 PTR-TOF-MS是第一次用于不同物种/类别的熟肉的快速和非侵入性剖析。特定VOC简档的知识铺平了新的途径,用于通过感官描述,在验证物种或识别异常或欺诈时进行特征物种。

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