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Rapid Profiling of the Volatilome of Cooked Meat by PTR-ToF-MS: Underlying Latent Explanatory Factors

机译:PTR-TOF-MS的熟肉挥发物的快速剖析:潜在的潜在解释因素

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摘要

Volatile organic compounds (VOCs) are important contributors to meat aroma and are variably correlated with each other. To study the sources of variation and the correlations among meat VOCs, meat cuts from five animal species/categories (chicken, turkey, pork, veal, and beef; two animals/species/retailer: 100 meat cuts) were obtained by 10 retailers. Each cut was processed into four burgers, two of which were grilled and two were cooked in a water bath (400 meat burgers). VOCs were detected by Proton-Transfer-Reaction Time-of-Flight Mass-Spectrometry (PTR-ToF-MS). From these, 129 peaks were selected, of which 72 were tentatively identified as relevant VOCs. Pearson correlations revealed a large number of positive and negative relationships among the VOCs. A multivariate statistical analysis revealed that 87% of the matrix covariance was explained by 17 independent Latent Explanatory Factors (LEFs), which have been described and characterized. LEFs identified may be valuable tools for reducing the dimensionality of results from VOC analyses and can be useful for better understanding and interpreting the variation in the meat aroma profile, although further study is required to characterize their sensory meaning.
机译:挥发性有机化合物(VOC)是肉类香气的重要贡献者,并且可变地与彼此相关。为了研究肉毒杆菌的变异来源和相关性,来自五种动物种类/类别的肉类(鸡,火鸡,猪肉,小牛肉和牛肉;两只动物/物种/零售商:100个肉切割)获得了10个零售商。将每条切割成四个汉堡,其中两种烧烤,两者在水浴(400肉汉堡)中煮熟。通过质子转移反应 - 飞行时间质谱(PTR-TOF-MS)检测VOC。从这些,选择129个峰,其中72次暂时被确定为相关VOC。 Pearson相关性揭示了VOC之间的大量积极和负面关系。多变量统计分析显示,通过描述和表征的17个独立潜在的解释因素(LEF)解释了87%的基质协方差。确定的lefs可能是降低VOC分析结果的重要工具,并且可以有助于更好地理解和解释肉类香气概况的变化,尽管需要进一步研究以表征其感官含义。

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