Photodynamic therapy induces autophagy-mediated cell death in human colorectal cancer cells via activation of the ROS/JNK signaling pathway

摘要

A HCT116 and SW480 cells were treated with various concentrations of m-THPC (0–12.0 μmol/L) or VP (0–6.0 μmol/L) for 8 h, respectively, and then irradiated with a light dose of 3 J/cm2, followed by incubation without irradiation for 24 h. Cell viability was detected by an MTT assay. B, C HCT116 and D, E SW480 cells were treated with m-THPC (final concentration, 0.7 μmol/L) or VP (final concentration, 0.35 μmol/L), respectively, for 8 h, and then irradiated with a light dose of 3 J/cm2, followed by incubation without irradiation for another 2, 4, and 6 h. ROS production in HCT116 and SW480 cells was detected by flow cytometry. All data are presented as the mean ± SD, n = 3. **P < 0.01, ***P < 0.001.