TROAP switches DYRK1 activity to drive hepatocellular carcinoma progression

摘要

A The mRNA expression correlations between TROAP and cell proliferation-associated genes in HCC tissues were analyzed using TCGA database. IHC staining with antibodies against TROAP and Ki67 were performed in serial sections from two HCC patients with low or high expression of TROAP (B), or from orthotopic xenograft tumors derived from vector or TROAP-transfected HepG2 and Huh7 cells (C), respectively. Scale bar, 200 μm. In C, normal liver tissues were indicated by asterisks. D The cell cycle distributions of Hep3B and PLC8024 cells with or without TROAP silence were analyzed by flow cytometry. One way ANOVA; **P < 0.01. E Western blotting was used to analyze the expressions of cell cycle-associated proteins in HepG2 and Huh7 cells transfected with vector or TROAP, and in Hep3B and PLC8024 cells with or without TROAP knockdown. β-Tubulin was also tested as a loading control.