Spatially and temporally defined H2O2 signatures are essential parts of various signaling pathways. Therefore, monitoring H2O2 dynamics with high spatio–temporal resolution is significantly important to understand how this ubiquitous signaling molecule can control diverse cellular responses. In this study, we designed and characterized a Fluorescence Resonance Energy Transfer (FRET)-based genetically encoded H2O2 sensor that provides a powerful tool to monitor the spatio–temporal dynamics of H2O2 fluxes. We have used this sensor to monitor the flux of H2O2 in live cells under stress conditions. Using this sensor, real-time information of the H2O2 level can be obtained non-invasively and would help to understand the adverse effect of H2O2 on cell physiology and its role in redox signaling.
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