BIOSENSOR for detecting hydrogen peroxide in living cells, possess enhanced resistance to change in pH

摘要

FIELD: medicine.;SUBSTANCE: invention relates to isolated nucleic acid, encoding protein with activity of fluorescent biosensor for hydrogen peroxide detection, expression vector, containing said nucleic acid, cell of E.coli, producing protein, encoded by said nucleic acid, and isolated protein, with activity of fluorescent biosensor for hydrogen peroxide detection. Isolated protein, with activity of fluorescent biosensor for hydrogen peroxide detection, has amino acid sequence, presented in form SEQ ID NO: 2. Expression vector contains nucleic acid, encoding protein with activity of fluorescent biosensor for hydrogen peroxide detection, under control of regulatory elements, necessary for expression of nucleic acid in host cell. Cell of E.coli is modifies due to introduction into it of nucleic acid, encoding protein with activity of fluorescent biosensor for hydrogen peroxide detection, functionally connected with regulatory sequence.;EFFECT: claimed invention is applied for detection of hydrogen peroxide inside live cells.;4 cl, 3 dwg, 3 ex