This study aimed to develop a nanoenzyme sensor that could detect hydrogen peroxide (H_2O_2) produced by cells, the graphdine materials were utilized due to its high specific surface area and good electrical conductivity additionly it could be loaded highly ordered porous materials of metal organic frameworks (MOFs). Due to the ability of the MoFs material to catalyze the oxidation of H_2O_2, the H_2O_2 produced by the cells was detected by amperometric method with the stimulation of the ascorbic acid. The electrochemical properties of the prepared graphdine-loaded MoFs were characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The high-sensitivity sensor was used to detect the content of H_2O_2 produced by living cells by amperometric method using a composite film of graphene@MoFs material to attach to the carbon fiber microelectrode.
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