Sensitivity spectra of mouse S- (magenta) and M-opsin (green) and rhodopsin (black; Rh), with emission spectra of UV (magenta, dotted) and green LED (green, dotted). Schematic distribution of cone photoreceptors across the mouse retina. Dots and shading represent distribution of true S-cones and co-expression ratio of S- and M-opsin in M-cones, respectively. d dorsal, n nasal, v ventral, t temporal. Schematic experimental setup for cone recordings. OS/IS outer/inner segment, ONL outer nuclear layer, OPL outer plexiform layer, INL inner nuclear layer, IPL inner plexiform layer, GCL ganglion cell layer. Red and yellow shading illustrate laser and stimulus beam, respectively. Example scan field (93 × 110 µm, 3.9 Hz) located in dorsal retina, showing iGluSnFR expression in the OPL (top, scale bar: 20 µm), correlation image (middle) and ROI mask (bottom). For display, the light artifact on the left side of scan fields was cut, resulting in 108 × 128 pixels (instead of 128 × 128). Cone responses of exemplary ROIs from ( , bottom) to full-field UV, green and white flashes (700 µm in diameter, scale bar: 1 s). As vertebrate photoreceptors are Off cells, light responses correspond to a decrease in glutamate release. Traces show mean glutamate release with s.d. shading ( > 10 trials, error bars: 1 s.d.). Dotted line indicates baseline. Traces scaled according to s.d. of baseline. Cells from ( , bottom) color-coded according to their SC in response to full-field flashes. Glutamate traces of cells from ( , bottom) in response to UV and green center (150 µm in diameter) and surround flashes (scale bar: 2 s). Cells from ( , bottom) color-coded based on center (left) and surround SC (right). Correlation image (top) and ROI mask (bottom) for an exemplary scan field located in the ventral retina. – Like ( – ), but for cells shown in ( , bottom). Scan fields/traces shown in this figure correspond to representative examples. In total, we recorded = 52 scan fields in = 9 mice. For quantification, see Fig. .
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