The NIN transcription factor coordinates CEP and CLE signaling peptides that regulate nodulation antagonistically

摘要

Expression analysis of genes in wild-type (WT) roots 1 day post rhizobial inoculation (1 dpi). qRT-PCR was used to measure the gene expression levels, normalized relative to uninoculated roots. To highlight fold changes, the dotted line corresponds to a ratio of 1. Data points from four biological replicates are plotted as open circles (  = 8). Expression analysis of genes in WT roots in response to a Nod factor (NF) 10  M treatment for 3 h. qRT-PCR was used to measure the expression levels in treated roots. The results were normalized relative to untreated roots. To highlight fold changes, the dotted line corresponds to a ratio of 1. Data points from six biological replicates are plotted as open circles (  ≥ 11). Expression analysis of genes in WT roots in response to a cytokinin (BAP 10  M) treatment for 3 h. qRT-PCR was used to measure the gene expression levels in treated roots. The results were normalized relative to untreated roots. To highlight fold changes, the dotted line corresponds to a ratio of 1. Data points from four biological replicates are plotted as open circles (  ≥ 8). In – , center lines show the medians; box limits indicate the 25th and 75th percentiles as determined by the R software; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles, outliers are represented by dots; and crosses represent sample means. Mann–Whitney test was used for each gene to assess significant differences between treated and control conditions, as indicated by asterisks (*  α α