Preparation and Characterization of a Polar Milk Lipid-enrichedComponent from Whey Powder

摘要

Milk fat globule membrane (MFGM) is a lipid carrier in mammals including humansthat consists mainly of polar lipids, like phospholipids and glycolipids. Inthis study, a process to enrich polar lipids in commercial butter and wheypowder, including polar lipids of MFGM, was developed. WPC (whey proteinconcentrate) 60 was selected as the most suitable raw material based on theyield, phospholipid, protein, and lactose content of the polar lipid fractionobtained by ethanol extraction of two WPC (WPC60 and WPC70) and two buttermilk(A and B). After fractionation under optimum conditions, the polar-lipidenriched fraction from WPC60 contained 38.56% phospholipids. The contentof glycolipids, cerebroside, lactosylceramide, ganglioside GM3, ganglioside GD3,was 0.97%, 0.55%, 0.09%, and 0.14%, respectively.Rancimat results showed that the oxidation stability of fish oil increased withan increase in the polar-lipid fraction by more than 30 times. In addition, thesecretion of IL-6 and TNF-α decreased in a concentration-dependent mannerafter treatment of RAW 264.7 cells with 0.1 to 100 ppm of the polar lipidfraction. In this study, polar lipid concentrates with antioxidant andanti-inflammatory activity, were prepared from milk processing by-products. TheMFGM polar lipid concentrates made from by-products are not only additives forinfants, but are also likely to be used as antioxidants in cooking oils and asactive ingredients for functional foods.