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Dataset on free amino acids contents of Serra da Estrela PDO cheeses determined by UPLC-DAD-MS/MS

机译:通过UPLC-DAD-MS / MS测定的塞拉达埃斯特雷拉PDO奶酪中游离氨基酸含量的数据集

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摘要

The composition of Serra da Estrela PDO cheeses (total fat, total protein, salt and free amino acids) was assessed using NIR spectrophotometry and UPLC-DAD-MS/MS. In total, 24 cheeses were acquired from 6 certified cheesemakers located in 5 different municipalities within the delimited PDO geographical region. Cheeses were produced from raw ewe milk of two autochthonous Portuguese sheep breeds, between November 2017 and March 2018, and were acquired after 45 days of maturation. The data include the mean (and respective standard deviations) levels of moisture (%), total fat (%), total protein (%) and salt (%), obtained by NIR spectrophotometry. As well the mean (and respective standard deviations) of free amino acids contents (mg/100 g of cheese, in wet basis) evaluated using a UPLC-DAD-MS/MS method are shown. The latter data include information regarding 8 essential free amino acids (histidine, leucine-isoleucine, lysine, methionine, phenylalanine, threonine, tryptophan and valine) and 9 non-essential free amino acids (arginine, asparagine, aspartic acid, cysteine, glutamine, glutamic acid, proline, tyrosine and serine). Leucine and isoleucine, being isomers, were quantified together. Leucine-isoleucine, phenylalanine and serine were the most abundant essential free amino acids and cysteine, proline and asparagine were the most abundant non-essential free amino acids. Free amino acids contents depended on the cheese producer as well as on the production time-period.
机译:使用NIR分光光度法和UPLC-DAD-MS / MS对Serra da Estrela PDO奶酪的成分(总脂肪,总蛋白质,盐和游离氨基酸)进行了评估。总共从定界的PDO地理区域内5个不同城市的6家获得认证的奶酪制造商那里购买了24种奶酪。奶酪是在2017年11月至2018年3月之间使用两种葡萄牙本土羊的原始母羊乳制成的,经过45天的熟化后获得。数据包括通过NIR分光光度法获得的水分(%),总脂肪(%),总蛋白质(%)和盐(%)的平均值(以及相应的标准偏差)。还显示了使用UPLC-DAD-MS / MS方法评估的游离氨基酸含量(以湿基计,mg / 100 g奶酪)的平均值(和相应的标准偏差)。后一种数据包括有关8种必需氨基酸(组氨酸,亮氨酸-异亮氨酸,赖氨酸,蛋氨酸,苯丙氨酸,苏氨酸,色氨酸和缬氨酸)和9种非必需氨基酸(精氨酸,天冬酰胺,天冬氨酸,半胱氨酸,谷氨酰胺,谷氨酸,脯氨酸,酪氨酸和丝氨酸)。亮氨酸和异亮氨酸是异构体,被一起定量。亮氨酸-异亮氨酸,苯丙氨酸和丝氨酸是最丰富的必需游离氨基酸,而半胱氨酸,脯氨酸和天冬酰胺是最丰富的非必需游离氨基酸。游离氨基酸的含量取决于奶酪生产商以及生产时间。

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