Downregulation of Siah1 promotes colorectal cancer cell proliferation and migration by regulating AKT and YAP ubiquitylation and proteasome degradation

摘要

Decreased expression of Siah1 indicates poor clinical prognosis in colorectal cancer. Expression of Siah1 protein in ten primary CRC (T) and adjacent noncancerous tissues (N) paired from the same patient, assessed by Western blot (left). The protein expression levels were quantified by comparing the gray level of each band using Quantity one Software (right). Average N/T ratio of Siah1 mRNA expression by RT-QPCR(n = 50). The expression of mRNA levels was normalized with GAPDH. Error bars represent mean ± SD calculated from 3 parallel experiments. Representative images of Siah1 expression in normal intestinal epithelium and CRC specimens with different differentiation examined by IHC. Siah1 was positively detected in normal intestinal epithelial cells (left), whereas it was only weakly (middle) or negatively (right) detected in CRC cells. Scale bar: 50 μm. Influence of Siah1 expression level on overall survival (upper left) and disease-free survival (upper right) of CRC patients by Kaplan–Meier analyses. Green, patients with low Siah1 expression (n = 115); blue, patients with high expression of Siah1 (n = 55). showed the effect of Siah1 expression level on overall survival of CRC patients(lower)