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De novo assembly of the olive fruit fly (Bactrocera oleae) genome with linked-reads and long-read technologies minimizes gaps and provides exceptional Y chromosome assembly

机译:橄榄果蝇(Bactrocera oleae)基因组的从头组装采用链读和长读技术可最大程度地减少缺口并提供出色的Y染色体组装

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摘要

Schematic of the method used to generate the different assemblies. DNA extracted from adult female and/or male insects was used to generate sequencing libraries for; Illumina paired-end (PE, 64X and 6X coverage, respectively), mate-pair (MP, 100X coverage), 10x Genomics linked-reads (100X coverage generated but 74X was found optimal for genome assembly), Pacific Biosciences (PacBio, 20X coverage), and Oxford Nanopore Technologies (ONT, 28X coverage). Independently generated assemblies are shown, and assemblies generated from scaffolding and gap scaffolding are shown with their GenBank accession numbers. Arrows indicate the final resulting assemblies while arrow heads indicate the samples or datasets used to generate the final assemblies
机译:用于生成不同程序集的方法的示意图。从成年雌性和/或雄性昆虫中提取的DNA用于生成测序文库。 Illumina的双末端(分别为PE,64X和6X覆盖),伴侣对(MP,100X覆盖),10x基因组链接读(生成1​​00X覆盖,但发现74X最适合基因组组装),Pacific Biosciences(PacBio,20X )和牛津纳米孔技术(ONT,28倍覆盖率)。显示了独立生成的程序集,并显示了从脚手架和间隙脚手架生成的程序集及其GenBank登录号。箭头指示最终的装配结果,而箭头指示用于生成最终装配的样本或数据集

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