An outbreak of Vibrio parahaemolyticus gastroenteritis on Canada’s west coast in 1997 emphasized the need to develop molecular methods for differentiation and typing of these organisms. Isolates were analyzed by enterobacterial repetitive intergenic consensus sequence (ERIC) PCR, detection of restriction fragment length polymorphisms (RFLP) in rRNA genes (ribotyping), pulsed-field gel electrophoresis (PFGE), and RFLP analysis of the genetic locus encoding the polar flagellum (Fla locus RFLP analysis). ERIC PCR and ribotyping were the most informative typing methods, especially when used together, while Fla locus RFLP analysis was the least discriminatory. PFGE exhibited good discrimination but suffered from a high incidence of DNA degradation. ERIC PCR and ribotyping will be useful for the evaluation of genetic and epidemiological relationships among V. parahaemolyticus strains.
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机译：1997年在加拿大西海岸爆发的副溶血性弧菌肠胃炎，强调需要开发分子方法来区分和区分这些生物。通过肠细菌重复基因间共有序列（ERIC）PCR，rRNA基因中的限制性片段长度多态性（RFLP）检测（核糖体分型），脉冲场凝胶电泳（PFGE）以及编码极性鞭毛的遗传基因座的RFLP分析来分析分离株。 （Fla基因座RFLP分析）。 ERIC PCR和核糖分型是最有用的分型方法，尤其是一起使用时，而Fla位点RFLP分析的鉴别性最低。 PFGE表现出良好的辨别力，但DNA降解的可能性很高。 ERIC PCR和核糖分型将有助于评估副溶血性弧菌菌株之间的遗传和流行病学关系。
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