The Isolator 10 lysis-centrifugation blood culture system (E. I. du Pont de Nemours & Co., Inc., Wilmington, Del.) was compared with the BACTEC radiometric method (Johnston Laboratories, Inc., Towson, Md.) with 6B and 7D broth media for the recovery of bacteria and yeasts. From 11,000 blood cultures, 1,174 clinically significant organisms were isolated. The Isolator system recovered significantly more total organisms, members of the family Enterobacteriaceae, Staphylococcus spp., and yeasts. The BACTEC system recovered significantly more Pseudomonas spp., Streptococcus spp., and anaerobes. Of the Isolator colony counts, 87% measured less than 11 CFU/ml of blood. Organisms, on an average, were detected the same day from each of the two culture systems. Only 13 of the 975 BACTEC isolates (0.01%) were recovered by subculture of growth-index-negative bottles, and 12 of the 13 were detected in another broth blood culture taken within 24 h. Contaminants were recovered from 4.8% of the Isolator 10 and 2.3% of the BACTEC cultures.
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机译：将Isolator 10裂解离心血液培养系统（EI du Pont de Nemours＆Co.，Inc.，特拉华州威尔明顿）与BACTEC放射法（Johnston Laboratories，Inc.，Towson，MD）进行了6B和7D的比较培养基用于细菌和酵母的回收。从11,000种血液培养物中分离出1,174种具有临床意义的生物。隔离器系统回收了更多的总生物，肠杆菌科，葡萄球菌属和酵母菌。 BACTEC系统显着恢复了更多的假单胞菌属，链球菌属和厌氧菌。在隔离菌的菌落计数中，有87％的人测得的血液低于11 CFU / ml。平均而言，同一天从两种培养系统中分别检测到生物。 975个BACTEC分离株中只有13个（0.01％）是通过生长指数阴性瓶的亚培养回收的，而13个中的12个是在24小时内采集的另一种肉汤血培养物中检测到的。从隔离器10的4.8％和BACTEC培养物的2.3％中回收了污染物。
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