Body Composition and Hormonal Effects Following Exposure to Mycotoxin Deoxynivalenol in the High Fat Diet-Induced Obese Mouse

摘要

The effects of ingesting the common foodborne mycotoxin deoxynivalenol (DON) on body weight and composition were characterized in the high fat (HF) diet-induced obese mouse, a model of human obesity. Female B6C3F1 mice were initially fed HF diets containing 45% kcal (HF45) or 60% kcal (HF60) as fat for 94 d to induce obesity. Half of each group was either continued on unamended HF diets or fed HF diets containing 10 mg/kg DON (DON-HF45 or DON-HF60) for another 54 d. Additional control mice were fed a low fat (LF) diet containing 10% kcal as fat for the entire 148 d period. DON induced rapid decreases in body weights and fat mass, which stabilized to those of the LF control within 11 d. These effects corresponded closely to a robust transient decrease in food consumption. While lean body mass did not decline in DON-fed groups, further increases were suppressed. DON exposure reduced plasma insulin, leptin, insulin-like growth factor 1 and insulin-like growth factor acid labile subunit as well as increased hypothalamic mRNA level of the orexigenic agouti-related protein. Overall, DON-mediated effects on body weight, fat mass, food intake and hormonal levels were consistent with a state of chronic energy restriction.