Serum dyslipidemia is induced by internal exposure to strontium-90 in mice lipidomic profiling using a data-independent LC/MS approach

摘要

Despite considerable research into the environmental risks and biological effects of exposure to external beam gamma rays, incorporation of radionuclides has largely been understudied. This dosimetry and exposure risk assessment is challenging for first responders in the field during a nuclear or radiological event. Therefore, we have developed a workflow for assessing injury responses in easily obtainable biofluids, such as urine and serum, as the result of exposure to internal emitters Cesium-137 (¹³⁷Cs) and Strontium-90 (⁹⁰Sr) in mice. Here we report on the results of the untargeted lipidomic profiling of serum from mice exposed to ⁹⁰Sr. We also compared these results to those from previously published ¹³⁷Cs exposure to determine any differences in cellular responses based on exposure type. The results of this study conclude that there is a gross increase in the serum abundance of triacylglycerides and cholesterol esters, while phostaphatidylcholines and lysophosphatidylcholines displayed decreases in their serum levels post exposure at study days 4, 7, 9, 25, and 30, with corresponding average cumulative skeleton doses ranging from 1.2±0.1 Gy to 5.2±0.73 Gy. The results show significant perturbations in serum lipidome as early as 2 days post exposure and persisting until the end of the study (day 30).