A comprehensive data-independent lipidomic survey in serum after chronic in-vivo exposure to the injury agent Strontium-90

摘要

Recent advances in the use of lipidomics in elucidating cellular signaling has utility is the assessment of injury responses as to ionizing radiation. Traditionally, most studies on radiation-induced cellular injury responses focused on changes in the gene expression and DNA damage repair machinery. However the field was revolutionized in the early 2000's by the application of mass spectrometry-based metabolomics, and more recently lipidomics1,2. Our lab has employed the use of shotgun lipidomics to characterize the global lipidomic signature for exposure by Strontium-90 (90Sr) in-vivo. Our untargeted lipidomic approach carried out using an ultra-performance liquid chromatography (UPLC)-time of flight mass spectrometer (TOFMS) was based on dataindependent acquisition mode, MSE, enabling us to rapidly identify different classes of lipids along with their oxidative fragments and lipid peroxides. This allowed us to develop a robust serum lipidomic profile for 90Sr exposure. This study demonstrates the power and speed of UPLC/TOFMSE in analyzing complex biofluids, and provided a panel of lipids, which may serve as potential serum markers to 90Sr exposure.