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Control of aflatoxigenic strains by Cinnamomum porrectum essential oil

机译:肉桂香精油控制黄曲霉毒株

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摘要

Essential oil from the bark of Cinnamomum porrectum (Roxb.) Kosterm was obtained by hydrodistillation using a Clevenger-type system for 3 h and identified by gas chromatography–mass spectrometry. The compounds were safrole (93.9%), elemicine (4.3%) and methyl eugenol (1.7%). The effect of essential oil on mycelial growth, sporulation, and aflatoxin B1 production of Aspergillus parasiticus IMI 283883 and Aspergillus flavus IMI 242684 was evaluated using contact and vapor treatments. Aflatoxin B1 was determined using the Enzyme-linked immunosorbent assay. The results showed that C. porrectum (Roxb.) Kosterm essential oil at concentrations more than 200 ppm exhibited inhibition effect on mycelial growth, sporulation, and aflatoxin B1 production of both Aspergillus strains as compared with control. The fumigation activities via vapor treatment showed higher inhibition than contact treatment. This study suggests that C. porrectum (Roxb.) Kosterm essential oil represents a good alternative in eco-friendly control of aflatoxigenic strain on food and agricultural commodities.
机译:通过使用Clevenger型系统加水蒸馏3小时,从加纳肉桂(Cinnamomum porrectum)(Roxb。)Kosterm的树皮中提取精油,并通过气相色谱-质谱法进行鉴定。化合物为黄樟脑(93.9%),艾美碱(4.3%)和丁香酚(1.7%)。使用接触和蒸汽处理评估了精油对寄生虫曲霉IMI 283883和黄曲霉IMI 242684的菌丝生长,孢子形成和黄曲霉毒素B1产生的影响。使用酶联免疫吸附测定法测定黄曲霉毒素B1。结果表明,与对照相比,浓度超过200 ppm的porcum porxum(Roxb。)Kosterm精油对两种曲霉菌株的菌丝生长,孢子形成和黄曲霉毒素B1产生均具有抑制作用。通过蒸气处理的熏蒸活性显示出比接触处理更高的抑制作用。这项研究表明,C。porrectum(Roxb。)Kosterm精油是食品和农产品中黄曲霉毒素菌株的生态友好控制的良好替代品。

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