PRDM5 promotes the proliferation and invasion of murine melanoma cells through up‐regulating JNK expression

摘要

PRDM (PRDI‐BF1 and RIZ domain‐containing) proteins constitute a family of zinc finger proteins and play important roles in multiple cellular processes by acting as epigenetic modifiers. PRDM5 is a recently identified member of the PRDM family and may function as a tumor suppressor in several types of cancer. However, the role of PRDM5 in murine melanoma remains largely unknown. In our study, effect of PRDM5 on murine melanoma cells was determined and results showed that PRDM5 overexpression significantly promoted proliferation, migration, and invasion of murine melanoma B16F10 cells. Consistently, silencing of PRDM5 expression significantly inhibited proliferation, invasion, and migration of B16F10 cells. In vivo study also showed that PRDM5 silencing significantly inhibited the growth and metastasis of melanoma in mice. PRDM5 was then found to increase the expression and activation of JNK in B16F10 cells. JNK silencing significantly reduced PRDM5‐mediated up‐regulation of JNK expression and blocked the style="fixed-case">PRDM5‐induced proliferation and invasion of B16F10 cells. To further verify the involvement of style="fixed-case">JNK signaling in style="fixed-case">PRDM5‐induced progression of B16F10 cells, a specific style="fixed-case">JNK inhibitor was employed to inhibit the style="fixed-case">JNK signaling pathway, and results showed that style="fixed-case">PRDM5‐induced proliferation and invasion of B16F10 cells were abolished. We conclude that style="fixed-case">PRDM5 promotes the proliferation and invasion of murine melanoma cells through up‐regulating style="fixed-case">JNK expression and strategies targeting style="fixed-case">PRDM5 may be promising for the therapy of melanoma.