Isolation identification of a laccase-producing fungal strain and enzymatic properties of the laccase

摘要

A new type of thermostable laccase was isolated from Paraphoma sp. GZS18, and its partial enzymatic properties were determined. A strain GZS18 of laccase with high yield was screened from forest soil and identified as Paraphoma sp. GZS18 through morphological characteristics and ITS sequence analysis. The laccase of Paraphoma sp. GZS18 (Lac-P) was obtained through cation–anion exchange chromatography, gel filtration chromatography, and other purification processes. The testing result shows that Lac-P is a single protein of 75 kDa, and the 11 amino acid sequences in the N-terminal are AX^aVSVASREMT (X^a was the non-standard protein). The optimum temperature and optimum pH of lac-P activity are substrate-independent. The temperature is in the range of 50–70 °C, and pH has high catalytic efficiency in the acidic range. Lac-P has good stability in the temperature and pH. The half time at 70–60 °C is 1.5 and 4 h, respectively. At pH 6–9 and room temperature, there is more than 80% activity 24 h later. Lac-P is tolerant of most metal ions and low concentrations of inhibitors but is inhibited by Hg²⁺, Fe²⁺ and NaN3. The laccase from Paraphoma sp. GZS18 at high temperature and pH 6–9, with strong stability, has better industrial application characteristics.