Construction and model-based analysis of a promoter library for E. coli: an indispensable tool for metabolic engineering

摘要

BackgroundNowadays, the focus in metabolic engineering research is shifting from massive overexpression and inactivation of genes towards the model-based fine tuning of gene expression. In this context, the construction of a library of synthetic promoters of Escherichia coli as a useful tool for fine tuning gene expression is discussed here.