Plasmids for Genome Engineering of E. coli and Method for Engineering Genome of E. coli Using Same

摘要

The present invention relates to a plasmid for engineering E. coli genomic DNA, an integrated plasmid for integrating a heterologous gene into E. coli genome, E. coli transformed with the plasmid, and a genome engineering method for wild-type E. coli using the plasmid, The plasmid has high portability to different E. coli strains, and the E. coli transformed with the plasmid exhibits high genome engineering efficiency and, through engineering, facilitates the recovery of an incorrectly mated repair system (MMR system) Which is very useful for engineering genomes in various synthetic biology and metabolic engineering fields.