High-level extracellular production of recombinant nattokinase in Bacillus subtilis WB800 by multiple tandem promoters

摘要

BackgroundNattokinase (NK), which is a member of the subtilisin family, is a potent fibrinolytic enzyme that might be useful for thrombosis therapy. Extensive work has been done to improve its production for the food industry. The aim of our study was to enhance NK production by tandem promoters in Bacillus subtilis WB800.