One-step triplex PCR/RT-PCR to detect canine distemper virus canine parvovirus and canine kobuvirus

摘要

To rapidly distinguish Canine distemper virus (CDV), canine parvovirus (CPV), and canine kobuvirus (CaKoV) in practice, a one-step multiplex PCR/RT-PCR assay was developed, with detection limits of 10^2.1 TCID50 for CDV, 10^1.9 TCID50 for CPV and 10³ copies for CaKoV. This method did not amplify nonspecific DNA or RNA from other canine viruses. Therefore, the assay provides a sensitive tool for the rapid clinical detection and epidemiological surveillance of CDV, CPV and CaKoV in dogs.