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Detection of Rare Mutations in EGFR-ARMS-PCR-Negative Lung Adenocarcinoma by Sanger Sequencing

机译：桑格测序法检测EGFR-ARMS-PCR-阴性肺腺癌中的罕见突变

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摘要

PurposeThis study aimed to identify potential epidermal growth factor receptor (EGFR) gene mutations in non-small cell lung cancer that went undetected by amplification refractory mutation system-Scorpion real-time PCR (ARMS-PCR).

机译：目的本研究旨在鉴定非小细胞肺癌中潜在的表皮生长因子受体（EGFR）基因突变，该突变未通过扩增难治性突变系统Scorpion实时PCR（ARMS-PCR）检测到。

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期刊名称 Yonsei Medical Journal
作者
Chaoyue Liang; Zhuolin Wu; Xiaohong Gan; Yuanbin Liu; You You; Chenxian Liu; Chengzhi Zhou; Ying Liang; Haiyun Mo; Allen M. Chen; Jiexia Zhang;
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作者单位

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年(卷),期 2018(59),1
年度 2018
页码 13–19
总页数 7
原文格式 PDF
正文语种
中图分类基础医学;
关键词
Non-small cell lung cancer EGFR mutation Sanger sequencing ARMS;

机译：非小细胞肺癌;EGFR突变;Sanger测序;ARMS;

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专利

1. Detection of Rare Mutations in EGFR-ARMS-PCR-Negative Lung Adenocarcinoma by Sanger Sequencing [J] . Liang Chaoyue, Wu Zhuolin, Gan Xiaohong, Yonsei Medical Journal . 2018,第1期

机译：桑格测序法检测EGFR-ARMS-PCR-阴性肺腺癌中的罕见突变
2. Two-round coamplification at lower denaturation temperature-PCR (COLD-PCR)-based sanger sequencing identifies a novel spectrum of low-level mutations in lung adenocarcinoma. [J] . Li J, Milbury CA, Li C Human mutation . 2009,第11期

机译：在较低变性温度下的两轮共扩增-基于PCR（COLD-PCR）的Sanger测序鉴定了肺腺癌中新的低水平突变谱。
3. Comparison of high resolution melting analysis, pyrosequencing, next generation sequencing and immunohistochemistry to conventional Sanger sequencing for the detection of p.V600E and non-p.V600E BRAF mutations [J] . Michaela Angelika Ihle, Jana Fassunke, Katharina K?nig, BMC Cancer . 2014,第1期

机译：高分辨率熔解分析，焦磷酸测序，下一代测序和免疫组织化学与常规Sanger测序的比较，可检测p.V600E和非p.V600E BRAF突变
4. Utility of ICE COLD-PCR Coupled to Sanger Sequencing or Next Generation Sequencing for Increased Mutation Detection Sensitivity During Patient Monitoring from Circulating Free DNA [C] . Katherine Richardson, Filip Janku, Ben Legendre, Molecular Medicine TRI-CON. . 2014

机译：冰冷PCR的效用偶联至Sanger测序或下一代测序，用于增加患者监测患者循环自由DNA期间的突变检测敏感性
5. The characteristics of the course of the disease in lung adenocarcinoma in relation to activating mutations of the gene for epidermal growth factor receptor. [D] . Stanic, Karmen. 2015

机译：与表皮生长因子受体基因激活突变有关的肺腺癌疾病病程特征。
6. Two-round COLD-PCR-based Sanger sequencing identifies a novel spectrum of low-level mutations in lung adenocarcinoma [O] . Jin Li, Coren A. Milbury, Cheng Li, -1

机译：基于两循环的冷PCR的Sanger测序识别肺腺癌中的新型低水平突变谱
7. Comparison of EGFR mutation detection between the tissue and cytology using direct sequencing, pyrosequencing and peptide nucleic acid clamping in lung adenocarcinoma: Korean multicentre study [O] . Kyueng-Whan Min, Wan-Seop Kim, Se Jin Jang, 2015

机译：肺腺癌中的直接测序，焦磷酸和肽核酸夹持的组织与细胞学之间EGFR突变检测的比较：韩国多期学研究

Detection of Rare Mutations in EGFR-ARMS-PCR-Negative Lung Adenocarcinoma by Sanger Sequencing

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