Accurate detection of rare genetic mutations in next-generation sequencing

摘要

The present invention is a method for analyzing a target nucleic acid fragment, comprising, from 5 ′ to 3 ′, an overhang adapter region, a primer ID region, and a target-specific sequence region complementary to one end of the target fragment. Primer extension using a first oligonucleotide primer creates a first strand using one strand of the target as a template, optionally removes an unincorporated primer, the produced first A method comprising the steps of amplifying a target from a strand and producing an amplification product, as well as detecting the amplification product. Specific primers useful for such target analysis methods are also disclosed. [Selection] Figure 1