Utility of ICE COLD-PCR Coupled to Sanger Sequencing or Next Generation Sequencing for Increased Mutation Detection Sensitivity During Patient Monitoring from Circulating Free DNA

摘要

The ICE COLD-PCR (ICP) technology is capable of high sensitivity detection for both point mutations and indels through unbiased enrichment of relevant gene regions. Mutation detection in circulating cell-free DNA (cfDNA) is becoming increasingly important for monitoring treatment and resistance responses to various cancer drugs. Since the amount of cfDNA in plasma is limited, a methodology, such as ICP, that provides increased sensitivity for detecting these informative mutations is needed.