NANO-DETERMINISTIC LATERAL DISPLACEMENT ARRAY FOR NEXT-GENERATION SEQUENCING SAMPLE PREPARATION AND HIGH-SENSITIVITY DNA DETECTION

摘要

A method of purifying nucleic acid polymers for next generation sequencing includes loading a mixture of adapter ligated, fragmented nucleic acid polymers onto a nano-deterministic lateral displacement (nanoDLD) array. The mixture includes a first adapter ligated, fragmented nucleic acid polymer, a second adapter ligated, fragmented nucleic acid polymer, and a contaminant. The method further includes flowing the mixture through the nanoDLD array to separate the first adapter ligated, fragmented nucleic acid polymer from the second adapter ligated, fragmented nucleic acid polymer, and each of the first and second adapter ligated, fragmented nucleic acid polymers from the contaminant.