AUTOMATED SAMPLE PREPARATION PLATFORM FOR NEXT GENERATION DNA SEQUENCING USING A DIGITAL MICROFLUIDIC HUB

摘要

We have demonstrated a novel capillary interface enabling highly repeatable transfer of liquid between a digital microfluidic (DMF) platform, which functions as a central fluid distribution hub, and multiple sample processing subsystem modules. This unique capillary interface allows both continuous-flow and droplet-based sample manipulations to be performed in one integrated system for automating library preparation steps for next-generation sequencers. Here we describe the use of this capillary-to-DMF interface in conjunction with an in-line contactless conductivity detector to enable and automate the closed-loop fraction collection and sorting of analyte droplets delivered to the DMF device.