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Extracts of Opuntia humifusa Fruits Inhibit the Growth of AGS Human Gastric Adenocarcinoma Cells

机译:鹰嘴豆果实提取物抑制AGS人胃腺癌细胞的生长

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摘要

Opuntia humifusa (OHF) has been used as a nutraceutical source for the prevention of chronic diseases. In the present study, the inhibitory effects of ethyl acetate extracts of OHF on the proliferation of AGS human gastric cancer cells and the mode of action were investigated. To elucidate the antiproliferative mechanisms of OHF in cancer cells, the expression of genes related to apoptosis and cell cycle arrest were determined with real-time PCR and western blot. The cytotoxic effect of OHF on AGS cells was observed in a dose-dependent manner. Exposure to OHF (100 μg/mL) significantly induced (P<0.05) the G1 phase cell cycle arrest. Additionally, the apoptotic cell population was greater (P<0.05) in OHF (200 μg/mL) treated AGS cells when compared to the control. The expression of genes associated with cell cycle progression (Cdk4, Cdk2, and cyclin E) was significantly downregulated (P<0.05) by the OHF treatment. Moreover, the expression of Bax and caspase-3 in OHF treated cells was higher (P<0.05) than in the control. These findings suggest that OHF induces the G1 phase cell cycle arrest and activation of mitochondria-mediated apoptosis pathway in AGS human gastric cancer cells.
机译:鹰嘴豆(OHF)已被用作预防慢性疾病的营养来源。在本研究中,研究了OHF乙酸乙酯提取物对AGS人胃癌细胞增殖的抑制作用和作用方式。为了阐明OHF在癌细胞中的抗增殖机制,通过实时PCR和western blot检测与凋亡和细胞周期阻滞有关的基因的表达。以剂量依赖性的方式观察到OHF对AGS细胞的细胞毒性作用。暴露于OHF(100μg/ mL)会明显诱导(P <0.05)G1期细胞周期停滞。此外,与对照相比,在OHF(200μg/ mL)处理的AGS细胞中凋亡细胞群更大(P <0.05)。通过OHF处理,与细胞周期进程相关的基因(Cdk4,Cdk2和cyclin E)的表达显着下调(P <0.05)。此外,在OHF处理的细胞中,Bax和caspase-3的表达高于对照(P <0.05)。这些发现表明OHF诱导AGS人胃癌细胞中G1期细胞周期停滞和线粒体介导的凋亡途径的激活。

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