Incorporation of Labeled Precursors into the RNA and Proteins of Lactic Dehydrogenase Virus

摘要

Lactic dehydrogenase virus was grown in primary mouse embryo cells and labeled with ³H-uridine and ³H-amino acids. Concentrated and purified virus was banded by isopycnic centrifugation in sucrose gradients, and infectivity and radioactivity were found to correspond at a density of 1.17 g/cm³. The extracted viral RNA was resolved by electrophoresis in polyacrylamide-agarose mixed gels, and the mol wt was estimated to be 6.0 × 10⁶.