Improved sensitivity of trypan blue dye exclusion assay with Ni2+ or Co2+ salts

摘要

A modified trypan blue dye exclusion assay was developed usingNi²⁺ or Co²⁺ salts to determine the viability ofprimary and transformed cells. When the cells were preincubatedwith NiSO4 or CoCl2 followed by trypan blue assay, thecontrast between stained and unstained cells was significantlyincreased as compared to the conventional trypan blue dyeexclusion assay.