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The Application of Metagenomics to Study Microbial Communities and Develop Desirable Traits in Fermented Foods

机译:宏基因组学在发酵食品中研究微生物群落和开发理想特性的应用

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摘要

The microbial communities present within fermented foods are diverse and dynamic, producing a variety of metabolites responsible for the fermentation processes, imparting characteristic organoleptic qualities and health-promoting traits, and maintaining microbiological safety of fermented foods. In this context, it is crucial to study these microbial communities to characterise fermented foods and the production processes involved. High Throughput Sequencing (HTS)-based methods such as metagenomics enable microbial community studies through amplicon and shotgun sequencing approaches. As the field constantly develops, sequencing technologies are becoming more accessible, affordable and accurate with a further shift from short read to long read sequencing being observed. Metagenomics is enjoying wide-spread application in fermented food studies and in recent years is also being employed in concert with synthetic biology techniques to help tackle problems with the large amounts of waste generated in the food sector. This review presents an introduction to current sequencing technologies and the benefits of their application in fermented foods.
机译:发酵食品中存在的微生物群落是多样化和动态的,产生各种负责发酵过程的代谢物,赋予其独特的感官品质和促进健康的特性,并维持发酵食品的微生物安全性。在这种情况下,研究这些微生物群落以表征发酵食品和所涉及的生产过程至关重要。基于高通量测序 (HTS) 的方法(如宏基因组学)可通过扩增子和鸟枪法测序方法进行微生物群落研究。随着该领域的不断发展,测序技术变得越来越容易获得、负担得起和准确,并且正在从短读长测序进一步转变。宏基因组学在发酵食品研究中得到广泛应用,近年来还与合成生物学技术相结合,以帮助解决食品行业产生的大量废物问题。本文介绍了当前的测序技术及其在发酵食品中应用的好处。

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