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The high-throughput sequencing of small RNAs profiling in wide hybridisation and allopolyploidisation between Brassica rapa and Brassica nigra

机译:甘蓝型油菜和黑芥子之间的广泛杂交和同种多倍体化中小RNA谱的高通量测序

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摘要

Small RNAs play an important role in maintaining the genome reconstruction and stability in the plant. However, little is known regarding the role of small RNAs during the process of wide hybridisation and chromosome doubling. Therefore, the changes in the small RNAs were assessed during the formation of an allodiploid (genome: AB) and its allotetraploid (genome: AABB) between Brassica rapa (♀) and Brassica nigra (♂) in the present study. Here, the experimental methods described in details, RNA-seq data (available at Gene Expression Omnibus database under ) and analysis published by Ghani et al. [1]. The study showed that small RNAs play an important role in maintaining the genome stability, and regulate gene expression which induces the phenotype variation in the formation of an allotetraploid. This may play an important role in the occurrence of heterosis in the allotetraploid.
机译:小RNA在维持植物中的基因组重建和稳定性中起重要作用。但是,关于小RNA在广泛杂交和染色体加倍过程中的作用知之甚少。因此,在本研究中,在甘蓝型油菜(♀)和黑菜型油菜(between)之间形成同源二倍体(基因组:AB)和其异源四倍体(基因组:AABB)期间评估了小RNA的变化。在这里,详细描述了实验方法,RNA-seq数据(可从的Gene Expression Omnibus数据库中获得)和由Ghani等人发表的分析。 [1]。研究表明,小RNA在维持基因组稳定性中起重要作用,并调节基因表达,从而诱导异源四倍体形成中的表型变异。这可能在异源四倍体中杂种优势的发生中起重要作用。

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