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De Novo Transcriptome Sequencing of the Orange-Fleshed Sweet Potato and Analysis of Differentially Expressed Genes Related to Carotenoid Biosynthesis

机译:橙皮甘薯的从头转录组测序及与类胡萝卜素生物合成相关的差异表达基因分析

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摘要

Sweet potato, Ipomoea batatas (L.) Lam., is an important food crop worldwide. The orange-fleshed sweet potato is considered to be an important source of beta-carotene. In this study, the transcriptome profiles of an orange-fleshed sweet potato cultivar “Weiduoli” and its mutant “HVB-3” with high carotenoid content were determined by using the high-throughput sequencing technology. A total of 13,767,387 and 9,837,090 high-quality reads were produced from Weiduoli and HVB-3, respectively. These reads were de novo assembled into 58,277 transcripts and 35,909 unigenes with an average length of 596 bp and 533 bp, respectively. In all, 874 differentially expressed genes (DEGs) were obtained between Weiduoli and HVB-3, 401 of which were upregulated and 473 were downregulated in HVB-3 compared to Weiduoli. Of the 697 DEGs annotated, 316 DEGs had GO terms and 62 DEGs were mapped onto 50 pathways. The 22 DEGs and 31 transcription factors involved in carotenoid biosynthesis were identified between Weiduoli and HVB-3. In addition, 1,725 SSR markers were detected. This study provides the genomic resources for discovering the genes involved in carotenoid biosynthesis of sweet potato and other plants.
机译:甘薯番薯(Ipomoea batatas(L.)Lam。)是全球重要的粮食作物。橙皮甘薯被认为是β-胡萝卜素的重要来源。在这项研究中,通过使用高通量测序技术确定了橙肉甘薯品种“ Weiduoli”及其具有高类胡萝卜素含量的突变体“ HVB-3”的转录组谱。分别从Weiduoli和HVB-3产生了13,767,387和9,837,090高质量的读段。这些读物从头开始组装成58,277个转录本和35,909个单基因,平均长度分别为596 bp和533 bp。在魏多里和HVB-3之间共获得874个差异表达基因(DEG),与魏多里相比,其中401个被上调,而473个被下调。在697个带注释的DEG中,有316个具有GO术语,而62个DEG被映射到50个路径上。在Weiduoli和HVB-3之间鉴定了参与类胡萝卜素生物合成的22个DEG和31个转录因子。此外,还检测到1,725个SSR标记。这项研究为发现甘薯和其他植物的类胡萝卜素生物合成相关基因提供了基因组资源。

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