三七总皂苷对HB-EGF诱导的系膜细胞增殖作用研究

摘要

目的：观察三七总皂苷对肝素结合性表皮生长因子样生长因子（HB-EGF）诱导大鼠肾小球系膜细胞增殖的作用。方法：以原代培养大鼠肾小球系膜细胞为细胞模型，以400ng/mL三七总皂苷和100ng/mL HB-EGF为干预因素，实验分对照组、100ng/mL HB-EGF组和400ng/mL三七总皂苷+100ng/mL HB-EGF组，刺激系膜细胞24、36、48h，采用MTT方法，观察三七总皂苷对重组HB-EGF诱导系膜细胞增殖的作用情况。结果：HB-EGF刺激36h时，系膜细胞明显增殖，与对照组比较，P<0.05；三七总皂苷能抑制HB-EGF诱导的系膜细胞的增殖，与HB-EGF组比较，P<0.05。随着刺激时间的延长，三七总皂苷对HB-EGF诱导系膜细胞增殖的抑制作用增强，但36h与24h比较，差异无统计学意义（P>0.05）；与48h比较，差异有统计学意义（P<0.05）。结论：三七总皂苷能抑制HB-EGF诱导的系膜细胞增殖作用，且作用随着刺激时间延长而增强。三七总皂苷可能通过抑制HB-EGF对系膜细胞增殖作用而延缓肾小球硬化。%Objective:To investigate the influence of panax notoginseng saponins (PNS) on proliferation of mesangial cell induced by heparin-binding epidermal growth factor like growth factor(HB-EGF) and its mechanism in kidney disease. Methods:The primary cultured SD rat mesangial cell were used as cell models. The cells were divided into control group,100 ng/ml HB-EGF group,and 400 ng/ml PNS+100 ng/ml HB-EGF group,and were cultured with these solutions for 24,36,and 48 h,respectively. Cell proliferation was assessed by MTT colorimetric assay. Results:HB-EGF stimulated the proliferation of mesangial cells in 36 h (compared with control group P<0.05)but the effect was inhibited by PNS (compared with HB-EGG group P<0.05),and the effect of inhibition in 48 h was more obvious than that in 36 h(P<0.05). But no significant difference in the effect of inhibition was noted between cells treated for 36 h and 24 h (P>0.05). Conclusion:PNS can inhibit the proliferation of mesangial cells induced by HB-EGF,and the effect of inhibition gradually increases with the prolongation of the culture time. PNS may re-tard the development of glomerular sclerosis by inhibiting the proliferation of mesangial cells induced by HB-EGF.