啶氧菌酯原药中主要杂质的制备、鉴定和定量分析

摘要

Picoxystrobin is a kind of fungicides belonging to strobilurin group. In this article, a main impurity of picoxystrobin was prepared and analyzed. The impurity was firstly identified by the liquid chromatography-mass spectrometry (LC-MS) and the content of the impurity was quantified by the liquid chromatography. The recovery of the quantitative analysis method was 99.13%, the linear range was 8.2~164.9 mg/L, the coefficient of determination was 0.997 5. The preparation and analysis method of the main impurity of picoxystrobin was established.%建立了啶氧菌酯杂质制备和分析的方法。采用酯交换方法合成杂质，并通过柱层析法提纯，采用液相-质谱联用和液相色谱方法对啶氧菌酯杂质进行定性和定量分析。制备了啶氧菌酯原药中的主要杂质标准品，含量为97.0%。建立了啶氧菌酯主要杂质的定量分析方法，添加回收率为99.13%。当杂质的浓度为8.2~164.9 mg/L时，方法的线性相关系数为0.9975。本方法建立了啶氧菌酯原药中主要杂质的制备、定性和定量的分析方法，为啶氧菌酯原药质量分析和控制提供了依据。