目的：研究不同浓度的血清素对体外培养大鼠成骨细胞分化的影响。方法：分离培养新生SD大鼠颅骨的成骨细胞，分别向培养液中加入不同浓度的血清素，分为4组：0 mol/L组(空白对照组)、10-9 mol/L组、10-8 mol/L组、10-7 mol/L组，通过RT-PCR检测骨钙素（OCN），碱性磷酸酶( ALP)，Runt相关转录因子-2（Runx 2）和Osterix mRNA相对表达量的变化。结果：不同浓度的血清素均可以抑制骨钙素（OCN），碱性磷酸酶( ALP)，Runt相关转录因子-2（Runx 2）和Osterix mRNA的相对表达量，且这种抑制作用随着血清素浓度的增大而增强。结论：血清素可以剂量依赖性地抑制成骨细胞的分化，这为骨质疏松症的治疗提供了新的思路。%Objective: to study the effects of serotonin at different concentrations on differentiation and of cultured primary rat osteoblasts in vitro. Methods: the osteoblasts from the skull of newborn SD rats, were divied into four groups by the culture medium with different serotonin concentrations: 0mol/L group(blank control), 10-9mol/L group, 10-8mol/Lgroup and 10-7mol/L group. In addition, mRNA expression of osteocalcin(OCN), alkaline phosphatase(ALP), runt-related transcription factor2 (Runx2) and Osterix were quantitated by real-time reverse transcription polymerase chain reaction. Results: different concentrations of serotonin inhibited the mRNA expression of OCN, ALP, Runx2and Osterix of osteoblasts; And, the inhibitory effect of serotonin was dose-dependent. Conclusion:serotonin can inhibit differentiation of osteoblasts; which provide a novel approach for the treatments of osteoporosis.
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