星点设计-效应面法优化玄参提取工艺

摘要

目的:优选玄参中哈巴苷和哈巴俄苷的提取工艺.方法:采用星点设计-效应面法,以哈巴苷与哈巴俄苷总提取率为指标,考察提取溶剂、液料比和提取时间对其的影响.结果:18%~32%乙醇,液料比20∶.1~26∶.1,提取30~48min时玄参总提取率最高.结论:星点设计-效应面法优化了玄参中哈巴苷与哈巴俄苷的提取工艺,预测性良好且简便可行.%Objective: To optimize the; extraction process for radix scrophnlariae by central composite design and response surface methodology. Methods: Central composite design and response surface methodology was used to optimize the extracting process. The main influential factors of extraction efficiency are ethanol concentration,solvent ratio and reflux time;. The; conditions of the extraction were evaluated by the total content of harpagide and harpagoside. Results: Analysis indicated the optimum conditions of harpagide and harpagoside are 18% -32% ethanol,20 -26 fold solvent,ultrasonic extraction 30 -48 minutes. Conclusion:It shows that the optimum process for