多时间点观察电针'委中'对大鼠腰多裂肌损伤后IGF1R和IGFBP3的表达

摘要

Objective:To observe the effects of electro-acupuncture at BL 40 on the expression of the type 1 IGF receptor (IGF1R)and IGF binding protein 3(IGFBP3)in rats with multifidus muscle injury.Methods:A total of 90 male Sprague Dawley rats were randomly divided into blank group(BG),model group(MG)and BL 40 electro acupuncture group(WG),with 30 rats in each group.Then the rats in MG and WG were injected with 0.5% bupivacaine into both sides of L 4-5multifidus muscle.After model establishment,the rats in WG accepted electro-acupuncture once a day.The changes of IGF1R and IGFBP3 in 3 groups' multifidus muscle were observed and analyzed at 1st,2nd,3rd,7th and 14th day.Results:The expression of IGF1R at day 2,3,7, 14 was higher than BG(P<0.05),while WG was higher than MG at day 1 and day 2.The MG's expression of IGFBP3 was higher than BG's at day 7 and day 14.Compared with MG,the expression of IGFBP3 in WG decreased at day 2 and day 14.Conclusion:Electro-acupuncture at BL 40 could increase the expression of IGF1R and decrease the expression of IGFBP3,so as to promote the regeneration of tissue after multifidus muscle injury.%目的:研究多时间点电针"委中"穴对大鼠腰多裂肌损伤后1型胰岛素样生长因子受体(IGF1R)和胰岛素样生长因子结合蛋白3(IGFBP3)的表达.方法:选取90只雄性SD大鼠并将其随机分成空白组、模型组、电针委中组,每组30只,模型组和电针委中组腹腔麻醉后往双侧L4-5多裂肌注射0.5%布比卡因溶液制造多裂肌损伤模型,空白组不做处理,造模后电针委中组行1次/d电针委中穴治疗,3组分别于治疗后1 d、2 d、3 d、7 d、14 d后同步取材,通过Western Blotting法观察多裂肌中IGF1R、IGFBP3的表达动态变化.结果:治疗后2 d、3 d、7 d、14 d模型组IGF1R的表达高于空白组(P<0.05);治疗后1 d、2 d电针委中组IGF1R的表达高于模型组(P<0.05).治疗后7 d、14 d模型组IGFBP3表达高于空白组;治疗后2 d、14 d电针委中组IGFBP3表达低于模型组.结论:电针委中穴可在早期增加IGF1R的表达,并下调IGFBP3的表达,促进多裂肌损伤后的修复.