滩涂盐生植物盐角草对东海原甲藻生长的抑制效应

摘要

Phytoremediation, as one of the mechanisms for controlling marine algal blooms, is becoming an ecologically sustainable approach in recent years. In this work, we investigated the inhibitory effect of salt marsh halophyteSalicornia europaeaon Prorocentrumdonghaiense. Growth ofP.donghaiensewas not inhibited by the water extracts ofS.europaea root, but the culture filtrate of S.europaea and four organic extracts. The inhibitory of organic extracts was dose-dependent, and P.donghaiensewas most sensitive to the methanol extract. GC-MS analysis of the methanol extract revealed 27 lipophilic compounds, in which 2-furancarboxaldehyde, 5-(hydroxymethyl)-, n-hexadecanoic acid and 2-methoxy-4-vinylphenol were the most abundant components. Together, our results suggested that salt marsh halophyteS. europaeamight be applied as a new type material to control the growth ofP.donghaiense.%利用植物抑制藻类生长是目前海洋赤潮治理的有效生态手段之一。以东海原甲藻为材料，研究了滩涂盐生植物盐角草对东海原甲藻生长的抑制效应。结果显示：盐角草过滤培养液和4种根的有机相提取物（甲醇相、正丁醇相、氯仿相和石油醚相）能显著抑制东海原甲藻的生长，改变细胞大小。其中，石油醚相和正丁醇相提取物还能影响藻细胞Chla含量。有机相提取物中以甲醇相提取物的抑制效果最强，对盐角草甲醇相提取物进行GC-MS分析，得到27种化合物，含量最高的3个组分为5-羟甲基-2-呋喃甲醛、棕榈酸和4-乙烯基-2-甲氧基-苯酚。研究表明：滩涂盐生植物盐角草能够抑制东海原甲藻的生长，具备开发新型赤潮生物抑藻剂的潜力，有望应用于海洋赤潮的防治。