自制三清降糖缓释片对糖尿病兔血糖、血脂及氧化应激的影响

摘要

目的：探讨自制三清降糖缓释片对糖尿病兔血糖、血脂及体内氧化应激水平的影响。方法：采用高脂高糖饮食喂养健康雄性家兔4周后，静脉注射链脲佐菌素制备糖尿病模型。将造模成功的家兔随机分为正常组、模型组、格列美脲组、三清降糖方汤剂、三清降糖缓释片组5组，灌胃给药4周后，生化法测血脂、血糖浓度；放免法测血清胰岛素，并计算胰岛素抵抗指数；双抗体夹心-ELISA法测肿瘤坏死因子-α(TNF-α)；WST法测超氧化物歧化酶；比色法测丙二醛和谷胱甘肽。结果：糖尿病兔各给药组与用药前比较血糖、血脂及SOD值、血清胰岛素及其抵抗指数均有所下降(P＜0.05)，谷胱甘肽水平无变化（P＞0.05）；而MDA、TNF-α水平升高，各给药组比较无统计学差异（P＞0.05）。结论：三清降糖缓释片对糖尿病兔具有降血糖、降脂作用，同时能减轻氧化应激改变。%Objective: To explore the effects of self-made SanQing JiangTang sustained-release tablets on blood lipid, blood glucose and the level of oxidative stress of diabetic rabbits. Methods:Diabetic rabbit models were established by feeding them high fat and sugar diet and intravenous injection of streptozotocin. After the models were successfully made, the rabbits were randomized into the normal group, model group, glimepride group, San-Qing JiangTang prescription group and SanQing JiangTang sustained-release tablets group. After medicated for four weeks, the concentrations of blood lipid and blood glucose were detected by biochemical method;the insulin in the serum was measured by radioimmunoassay, insulin resistance index calculated;TNF-α with double antibody sand-wich ELISA method;SOD by WST method, MDA and glutathione (GSH) by chromatometry. Results:Blood glu-cose, blood lipid and SOD value were decreased when the medication groups after treating were compared with be-fore treating (P<0.05);the levels of MDA and TNF-αwere raised, there was no statistical differences between the medication groups (P>0.05). Conclusion:SanQing JiangTang sustained-release tablets could lower blood lipid and blood glucose, and it could relieve the changes of oxidative stress of diabetic rabbits.