RP-HPLC法测定防风通圣丸中栀子苷、芍药苷、黄芩苷、大黄素的含量

摘要

Objective: To establish the determination methods for the contents of jasminoidin, peoniflorin, baicalin and emodin inFangFengTongShengpills simultaneously by RP-HPLC. Methods: The contents were deter-mined under different wavelengths by adopting RP-HPLC as gradient elution. Chromatographic column: Zorbax E-clipse XDB-C18 bonded silica gel column (4.6 mm×150 mm, 5μm); column temperature was room temperature; mo-bile phase: acetonitrile-2% phosphoric acid aqueous solution, gradient elution was adopted; flow rate 1.0mL/min; de-tection wavelengths were 240nm (jasminoidin and peoniflorin) and 275nm (baicalin and emodin) respectively. Re-sults: Jasminoidin, peoniflorin, baicalin and emodin were in better linear relationship when their sample sizes were between 0.056 and 0.56g (r=0.999 7), 0.103 5 and 1.029 7g(r=0.999 2), 0.207 4 and 3.128 g(r=0.999 3), 0.04 and 0.423 g(r=0.999 6), average recovery rates were 99.14%, 99.65%, 99.03% and 99.16%; RSD was 1.08%, 1.01%, 0.85% and 0.86% respectively. Conclusion: The method, simple, rapid, sensitive, reproducible and of high precision and accurate results, could be used for content determination of jasminoidin, peoniflorin, baicalin and emodin in FangFengTongShengpills simultaneously.%目的：建立RP-HPLC同时测定防风通圣丸中栀子苷、芍药苷、黄芩苷和大黄素含量的方法。方法：采用高效液相色谱（RP-HPLC）梯度洗脱，在不同波长下测定含量。色谱柱：Zorbax Eclipse XDB-C18键合硅胶色谱柱(4.6 mm×150 mm,5μm)；柱温为室温；流动相为乙腈-2%磷酸水溶液，采用梯度洗脱；流速1.0 mL/min；检测波长分别为240 nm(栀子苷、芍药苷），275 nm（黄芩苷、大黄素）。结果：栀子苷、芍药苷、黄芩苷和大黄素的进样量分别在0.056～0.56 g(r=0.9997)，0.1035～1.0297 g(r=0.9992)，0.2074～3.128g（r=0.9993），0.04～0.423g（r=0.9996）范围内线性关系良好，平均加样回收率分别为99.14%，99.65%，99.03%，99.16%；RSD分别为1.08%，1.01%，0.85%,0.86%。结论：该方法简便、快速、灵敏、精密度高、重现性良好、结果准确，可用于同时测定防风通圣丸中栀子苷、芍药苷、黄芩苷和大黄素的含量。