糖尿病大鼠下颌骨成骨细胞葡萄糖转运蛋白-1及胰岛素受体α1的表达

摘要

目的 检测糖尿病大鼠下颌骨成骨细胞中葡萄糖转运蛋白(GLUT)-1、胰岛素受体(IR)α1的表达,探讨其对成骨细胞分化功能障碍的影响.方法 采用逆转录聚合酶链反应、蛋白质印迹分析、免疫组织化学染色观测糖尿病大鼠下颌骨成骨细胞及对照组细胞中GLUT-1、IR α1的表达.结果 糖尿病组GLUT-1及IR α1的mRNA表达均高于对照组,差异具有统计学意义(P<0.05).2细胞GLUT-1、IR α1的蛋白表达与其mRNA表达相似.结论 糖尿病大鼠下颌骨成骨细胞保留了对供体来源的高糖、低胰岛素环境作出的适应性变化,这可能是造成其分化功能障碍的原因之一.%Objective To explore the expression of glucose transporter(GLUT)-1 and insulin receptor(IR) α1 in osteoblast obtained from diabetic rats' mandibles. Methods The expression of GLUT-1 and IR α1 of diabetic and control groups were measured by reverse transcription polymerase chain reaction, Western blot and immunohistochemistry stain. Results The mRNA expressions of GLUT-1 and IR α1 of diabetic group were significantly higher than control group(P＜0.05). The protein expressions of GLUT-1 and IR α1 were similar to the mRNA expressions. Conclusion Osteoblasts obtained from diabetic rats' mandibles keep the adaptation changes to hyperglycemia and hypoinsulinemia,which may contribute to their dysfunction.