无透镜微流控成像流动细胞检测与计数系统

摘要

在未来面向个人化的生物医疗诊断中,实时的细胞检测与计数具有重要需求.现有的细胞检测和计数系统例如流式细胞仪和血细胞计数器不适用于小型化流动细胞实时检测和计数.通过将CMOS图像传感器芯片和微流控芯片结合,提出了一种用于流动细胞检测和计数的无透镜微流控成像系统,与用于计数静态细胞的其它无透镜微流控成像系统不同,该系统可以通过基于时域差分的运动检测算法检测和计数微流体通道中连续流动的细胞样本.测试结果表明:该系统可以对微流控通道中流动的人体骨髓基质细胞实现自动检测和计数,并具有-6.53％的低统计错误率.该系统提供了面向未来即时应用的细胞检测和计数解决方案.%The existing cell detection and counting systems such as flow cytometer and haemocytometer are not designed towards real-time for cells in flowing microfluid.Based on the integration of a CMOS image sensor and a microfluidic chip,a lensless microfluidic imaging system for detection and counting of flowing cells is presented.Different from the other lensless microfluidic imaging systems designed for counting static cells,the developed system can detect and count continuously flowing cells in microfluidic channel by temporal-differencing based motion detection algorithms.Measured experiment results show that human bone marrow stromal cells flowing in the microfluidic channel can be automatically detected and counted with low statistical error rate of-6.53 ％.The developed system thereby provides cell detection and counting solutions for point-of-care applications.