An immunoaffinity detection method for simultaneously determining the aflatoxins B1, B2, G1 and G2 in tobacco and tobacco products was developed via solvent extraction of sample, purifying and concentrating on immunoaffinity column, pre-column derivatization by trifluoroacetic acid, separation by HPLC, and detection by fluorescence detector. The results showed that: 1) The determination could be completed within 20 minutes, the four target aflatoxins were well separated and exhibited good linear relations with correlation coefficients r > 0.99. 2) The recoveries of the method ranged from 85% to 117%with the relative standard deviation (RSD) of 0.2%-9.4% (n=6), and the limits of detection and quantification of aflatoxin B1 were 0.10 and 0.34 μg/kg, respectively.%通过溶剂萃取、免疫亲和柱纯化富集、三氟乙酸柱前衍生、高效液相色谱(HPLC)法分离及荧光检测器检测,建立了同时测定烟草及烟草制品中黄曲霉毒素B1、B2、G1和G2的免疫亲和检测方法.结果表明:①该方法可在20 min内完成测定,4种目标物能够得到很好的分离,线性关系良好,相关系数r值均大于0.99.②方法的回收率为85%~117%,相对标准偏差为0.2%~9.4%(n=6),其中B1的检出限和定量限分别为0.10和0.34μg/kg.
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