烟草镉转运基因突变体的鉴定和功能分析

摘要

To obtain Cd-deficiency phenotype in tobacco, the mutant of NtHMA2 gene was screened by TILLING and the contents of Cd and other heavy metals in mutant were detected. The results showed that comparing with the control, the contents of Cd, Zn, Cr, Ni, As and Fe in mutant under Cd stress decreased by 36.84%, 27.92%, 32.48%, 30.18%, 11.33% and 30.51%, respectively. The plant height of mutant was reduced significantly. However, there was no significant difference of the other agronomic traits. The low Cd-content tobacco mutant obtained in this study, hma2-23, could be applied to develop anti-Cd tobacco varieties.%为获得对镉吸收能力下降的烟草材料,通过TILLING技术筛选出镉转运基因NtHMA2的突变体并测定了该突变体中镉及其他重金属的含量.结果表明,在镉胁迫下,与对照相比突变体烟叶中的镉含量降低36.84%;锌、铬、镍、砷、铁含量分别降低27.92%、32.48%、30.18%、11.33%、30.51%.突变体株高比对照显著降低,其他农艺性状无显著差异.获得的对镉吸收能力下降的突变体材料hma2-23可用于烟草抗镉胁迫品种的培育.